WebPNA-LNA PCR clamp analysis demonstrated that the EGFR mutation status of the primary lung cancer was identical to that of the intraductal tumor. EGFR del E746-A750 and … WebThe presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the …

Use of peanut agglutinin to assess the acrosomal status and

WebThe Germinal Center B Cell (PNA) MicroBead Kit, mouse has been optimized for the rapid and simple isolation of mouse germinal center B cells from single-cell suspensions from … WebCoomassie blue staining has been reported as an effective and inexpensive method for evaluating the acrosome integrity of spermatozoa, though to date its use to evaluate cryopreserved boar sperm has not been reported. Moreover, there is no information concerning the agreement between Coomassie blue staining and fluorescein … completely bony

Proteoglycan IMPG2 Shapes the Interphotoreceptor Matrix and …

WebSep 9, 2024 · Whereas a significant decrease in the Sia-binding lectin SNA was observed for GNEM-HEK cells, an inverse trend was observed for staining with PNA, a lectin that binds to nonsialylated structures (Figure 1b). These results confirm the efficacy of the interruption of Sia biosynthesis in the GNEM-HEK cells. WebMar 2, 2010 · The PNA staining outside the PNA-negative areas is similar to the retina of PD 10. The loss of PNA staining progresses with age (Fig. 3A–3F), as demonstrated clearly in the quantitative analysis of COS … WebAcrosome membrane integrity was evaluated following the protocol of ref. 54 , in which sperm were stained with PNA-FITC (final concentration: 1.2 µM) for 5 min at 38°C in the … ecabinet download