2024 Pbad-topo

Pbad-topo

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August undefined, 2024

Splet10. maj 2024 · pQE-30 编号载体名称北京华越洋生物VECT5540 pQE-30 pQE30 载体基本信息别名: pQE30, pQE 30 质粒类型: 大肠杆菌蛋白表达载体表达水平: 低拷贝启动子: T5 克隆方法: 多克隆位点，限制性内切酶载体大小: 3461 bp 5' 测序引物及序列: pQE30-F: AGCGGATAACAATTTCACACAG 3' 测序引物及 ... Splet免责声明: 本站发布的所有产品仅限于科研使用，不可用于临床的诊断和治疗。如有违反，与本站无关. 投诉、举报、维权邮箱：[email protected]

pBAD TOPO™ TA Expression Kit - Thermo Fisher Scientific

SpletpBAD-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning and arabinose-inducible expression. Sequence Author: Thermo … Splet23. mar. 2024 · 10115092 Invitrogen™ pBAD TOPO™ TA Expression Kit Specifically designed for one-step cloning and regulated prokaryotic expression of Taq -amplified PCR products Brand: Invitrogen™ K430001 Additional Details : Weight : 0.08000kg View more versions of this product Product Code. 10115092 1452.00 EUR / Each Estimated … smoke detector smart home

pCMVSPORT6CD_价格-品牌-详情介绍_丁香通

Splet丁香通为您提供pcmvsport6cd商品详情介绍：价格：￥14，货号：p44162，cas号：无，品牌：禾午生物，产地：国产，供应商：禾午生物，详见丁香通pcmvsport6cd商品详情页； SpletPlasmid pBAD-lacZ is used as an empty vector control for experiments performed in V. cholerae and has been described previously [37]. Plasmid pBAD-TOPO-NrnB-VSVG was generated by PCR amplification ... SpletProduct Features pBAD/TOPO® ThioFusion™ Expression Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq … smoke detectors in warehouses

pBAD Bacterial Recombinant Protein Expression Vector

MUC1 ectodomain is a flagellin-targeting decoy receptor and …

SpletpBAD202 D-TOPO. Bacterial directional TOPO® cloning vector for arabinose-inducible expression of solubilized and N- and C-terminally tagged proteins. Sequence Author: … Splet25. okt. 2024 · The membranes from V. natriegens pBAD-TOPO also showed NADH oxidation activity, due to the endogenous NQR (Figure 4 ). Nevertheless, a significant lower activity with ∼950 nmol min -1 mg -1 was observed, compared to V. natriegens pNqrST. riverside cafe crownSpletTOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is … riverside cafe chollerford

"Splet化工仪器网为您提供invitrogen 价格表【第15001条-第16000条】的详细介绍和价格，如有意向请联系上海起发实验试剂有限公司 " - Pbad-topo

Pbad-topo

Escherichiacoli YqhDExhibitsAldehydeReductaseActivity ...

SpletpBAD202/D-TOPO大肠杆菌阿拉伯糖诱导表达载体质粒-BioVector NTCC典型培养物保藏中心 - Biovector质粒载体菌种细胞蛋白抗体基因保藏中心-NTCC典型培养物保藏中心 pBAD202/D-TOPO载体质粒-BioVector NTCC典型培养物保藏中心首页 BioVector NTCC典型培养物保藏中心注册登录购物车 Biovector BioVector 质粒载体菌株细胞蛋白抗体基因保藏中心 … Spletcloned the LIC11037 gene using the pBad/thio-TOPO system (Invitrogen), expressed and purified from induced cultures through affinity chromatography (Ni2+-HisTrap). The recombinant protein showed the expected molecular mass and we are performing zymographic studies for characterization of this enzyme. Supported by: FAPERJ, CNPq.

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SpletpBAD-TOPO载体价格2000元，pBAD-TOPO载体质粒图谱(Vector map)，载体序列(Vector Sequence)见下文，载体质粒抗性为氨苄青霉素，质粒大小为4126bp，测序引物为pBAD … Splet30. jul. 2024 · pBAD/TOPO ThioFusion Expression Kit (Invitrogen). 2. Escherichia coli BL21 competent cells. 3. LB medium: Dissolve 5 g tryptone, 5 g NaCl, and 2.5 g yeast extract in 500 mL distilled water. Sterilize by autoclaving. 4. Ampicillin. 5. 20% l-Arabinose: Dissolve 2 g l-arabinose in 10 mL distilled water and sterilize with a 0.22 μm filter. 6.

Spletpbad/thio-topo载体是阿拉伯糖调控载体 ,在无葡萄糖的培养基中，阿拉伯糖正向调控目的基因的表达；通过调节阿拉伯糖的浓度水平来优化目的蛋白的可溶性表达；采用topo-ta技 … Splet01. apr. 2024 · We obtained pMC1403 (a promoterless reporter vector) (35) from the NIG and pBAD-TOPO/TA from Thermo Fisher Scientific (Waltham, MA, USA). Table 1 also summarizes the plasmids that were constructed in this study. We purchased the n-hexane and cyclohexane from FujiFilm Wako Pure Chemicals (Osaka, Japan).

SpletpBAD-TOPO® 4.1 kb P B A D A m p i c i l l i n pB R 3 2 2 o r i a r a C 1 Pme I RBS EK Site V5 epitope 6xHis Stop PCR Product Comments for pBAD-TOPO® 4126 nucleotides Note: … SpletThe pBAD vector system is a reliable and controllable system for expressing recombinant proteins in bacteria. This system is based on the araBAD operon, which controls E. coli L-arabinose metabolism. The gene of interest is placed into the pBAD vector downstream of the araBAD promoter, which drives expression of the gene of interest in response ...

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SpletIntroduction pBAD TOPO® TA Expression Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO ® Cloning") for the direct insertion of Taq polymerase- amplified … smoke detectors interconnected wireless smoke detectors in the kitchenSplet首页 / 专利分类库 / 一般的物理或化学的方法或装置 / 是有关分离的最通用的小类，但不包括从固体中分离出固体。 / 利用半透膜分离的方法，例如渗析，渗透，超滤；其专用设备，辅助设备或辅助操作 / ·超滤；微量过滤 / ··原料的预处理 / Lipid co-factor essential for … riverside cafe ellsworth maineSplet11. jul. 2024 · 1.表达宿主菌的选择. 大肠杆菌常用的表达宿主菌有E.ColiBL21(DE3),E.ColiRossatta(DE3),E.ColiBL21(DE3)Condon Plus。针对特殊的目标蛋白其所需要的宿主菌也不相同，每种宿主菌的基本特征可在本网站查询，福因德生物可提供表中所列菌种的感受态细胞，如需更多信息可联系福因德技术部门。 smoke detectors scdfSpletpET-30a大肠杆菌表达载体,质粒,菌株. pET-30a大肠杆菌表达载体,质粒,菌株_生物学_自然科学_专业资料。pET-30a大肠...操作说明: 1.请使用华越洋生物定制开箱器打开外包装; 2.该产品包含 20μl .... 大肠杆菌表达系统使用指导_图文. 大肠杆菌表达系统使用指导_自然科学_专业资料 … smoke detectors nhbcSpletTo characterize the structural and functional properties of viral interleukin 10 (vIL-10), its cDNA was cloned into the bacterial expression vector pMAL-c2, which directs the synthesis of the inserted gene as a fusion protein with maltose binding protein (MBP). smoke detector spray testerSplet02. jul. 2024 · E. coli DH5α and E. coli TOP10 were used as the hosts for the construction of plasmids derived from pMW218 and pBAD-TOPO, respectively. pBADacrABtolC, a pBAD-TOPO derivative containing acrAB and tolC cloned from E. coli W3110 , was constructed in this study. pMWxylMA, a pMW218 derivative containing xylMA, was also smoke detectors ionized or photoelectric