Web10 de fev. de 2024 · Long-lived protein degradation assay Cells were incubated for 36 h with 0.135 μCi/ml L-[ 14 C]valine supplemented complete RPMI (with 10% FBS and 1% penicillin and streptomycin) medium, followed by two washes and a 3 h chase for degradation of short-lived proteins in fresh medium containing 10 mM nonradioactive L … Web23 de set. de 2024 · Long-lived protein degradation assay. H4 cells were cultured with l-[3,4,5-3 H(N)]-leucine (0.1 μCi/ml) (PerkinElmer Life Sciences) for 24 h and chased in medium with nonradioactive leucine for …

The Long-lived Protein Degradation Assay: an Efficient …

Web24 de jun. de 2011 · Long-Lived Protein Degradation Assay EGF Receptor Degradation Assay MEF cells were incubated in serum-free DMEM for 3 hr, and then 3 μM thapsigargin (or DMSO control) was added to indicated plates for an additional 2 hr. Media was then replaced with serum-free DMEM containing 40 ng/ml EGF and 20 μg/ml cycloheximide, … Web5 de mai. de 2024 · The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent … cubist style picasso

Assay for proteasome-dependent protein degradation and …

WebWe next screened a collection of 160 cell-permeable protein kinase inhibitors in 96-well plates using the degradation assay (Fig. 3A and supplemental Table 3A). The rationale underlying this screen is that protein kinase inhibitors typically target the ATP-binding site, and we reasoned that some known kinase inhibitors might cross-inhibit one of the … Weblong-lived protein degradation, we also tested the effect of different nonradio-active chase durations to minimize the interference from short-lived proteins. As shown in Figure S2A, a longer chase time (2–12 h) markedly reduced the basal fluo - rescence level in the control group with-out starvation. Interestingly, as shown in WebAutophagy is a cellular process that long-lived proteins and damaged organelles are transported to lysosomes for degradation and reuse. 12 Besides inducing apoptosis, resveratrol can also induce autophagy in cancer cells. 16,23 Beclin1 is a specific marker of autophagy and is strongly involved in the autophagic process, especially in its early … mare fuori 4 quando iniziano le riprese