WebJan 1, 1988 · Studies of spinal drug action in mice often involve percutaneous intrathecal drug administration delivered in a lightly anesthetized animal. A successful lumbar intrathecal (IT) needle stick of a lightly anesthetized (isoflurane) mouse evokes a tail flick, which is an indication of local spinal nerve stimulation. WebMar 8, 2024 · The single intrathecal administration of CCL3 or CCL9 nAb, J113863, TAK-220, ... have prominent pronociceptive properties after their intrathecal administration to naive mice [13,16]. We were interested in three of the abovementioned chemokines with strong algesic potential belonging to the MIP-1 family: CCL3, CCL4, and CCL9.
Comparative Effectiveness of Intracerebroventricular, Intrathecal, …
WebOct 16, 2013 · The volume and the rate of delivery of PBS or MTX by mini-pump were ~100 nL per h continuously for 28 days. Mice received approximately 50 μg MTX per kg body weight and day mirroring the low dose intrathecal MTX administration in progressive MS patients (Sadiq et al., 2010). Mice were housed individually to avoid possible damage to … WebOct 17, 1980 · The present paper describes the injection technique, the distribution of intrathecal methylene blue dye and [3H/mor- phine, and the analgesic response of mice to intrathecally injected morphine sulfate. 2. Materials and methods Male Swiss-Webster mice (20~25 g, Biolab Inc., St. Paul, MN) were used throughout these experiments. marketsource employee login
Comparative Effectiveness of Intracerebroventricular, Intrathecal, …
WebIntracerebral (IC) injection of live rabies virus is an invasive and painful procedure. Appropriate IC injection techniques by trained personnel can minimize trauma associated with the technique. Technical guidance is available in several regulatory guidelines and publications ( Bruckner et al., 2003; USDA, 2007; Koprowski, 1996 ). WebIntrathecal delivery by direct lumbar puncture in conscious rodents offers a minimally invasive approach that avoids tissue damage and/or destruction. ... Detailed Method for … WebRemarkably, when allowed to resolve for several weeks, behavioral hypersensitivity could be dramatically reinstated with NPY knockdown or intrathecal administration of Y1 or Y2 receptor antagonists. marketsource customer service phone number