Hoechst 33342 work concentration
NettetWorking concentration Excitation filter (wavelength, nm) Hoechst 33342: 1 mM in distilled water: 1–10 μM: 350: Propidium iodide: 1 mg/ml in distilled water: 1–10 μg/ml: 535: … NettetHoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst 33342 exhibits a 10 fold greater cell …
Hoechst 33342 work concentration
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Nettet13. okt. 2024 · Hoechst 33342 is a cell-membrane permeant dye that reacts exclusively with double-stranded DNA, while Pyronin Y, also a cell-membrane permeant dye, reacts with DNA, RNA, and mitochondrial membranes. In the presence of Hoechst 33342, the Pyronin Y reaction with DNA is blocked and predominantly stains RNA when used at … NettetCharacteristics The blue fluorescent Hoechst 33258, 33342, 34580 and nuclear yellow dyes may be effi- ciently excited with a xenon or mercury-arc lamp or with a UV laser; …
NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used … NettetRecommended staining concentration: 1 ug/mL; Hoechst 33258, pentahydrate Hoechst 33342 Staining Protocol. λ Ex /λ Em (with DNA) = 350/461 nm; MW: 561.93 (in H 2 O); …
NettetHoechst 33342 will also work with parafomraldehyde or ethanol-fixed cells with modification to the above protocol. The reduction of Hoechst 33342 concentration … Nettet18. nov. 2024 · The absolute Hoechst concentration in the supernatant was calculated by comparison with samples containing known concentrations of Hoechst in Assay Buffer at fixed pH values between 6.0 and 9.0.
Nettet26. jun. 2007 · For Hoechst staining and side population analysis, cells were retrieved from subconfluent culture by trypsinization, resuspended at 10 6 cells/ml in prewarmed DMEM containing 2% FBS, 10 mM Hepes, then incubated for 90 min at 37 °C with Hoechst 33342 (5 μg/ml final concentration, Sigma) with intermittent mixing.
Nettetdone by applying Hoechst 33342 and PI in the following way: 2 ml of a harvested and PBS washed cell suspension (3 3 108 cells ml21) were incubated with 4 ll Hoechst 33342 for 35 min (final concentration: 3.24 lM). Then, 20 ll PIwere added and the sample analyzed after additional 10 min. Flow Cytometry and Cell Sorting Multiparametric flow ... colony theater shaker squareNettetHoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication. [ 2] Application A fluorescent DNA stain qualified for use in Mycoplama staining. Components colony tire corpNettet26. feb. 2024 · Hoechst 33342, 20 mM solution in water - 5 ml Labeling-detection Hoechst 33342, 20 mM solution in water - 5 ml €97.00 Check your price Cat.Number : AS-83218 Availability : In production Shipping conditions : Ice delivery fees must be applied Select specification Quantity Add to Cart Add to wishlist In Stock Reminder Overview … colony the tv seriesNettetfluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml. colony tire of new bernNettetHoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye. Optical Data for Hoechst 33342 Technical Data for Hoechst 33342 The … dr scholl\u0027s stores locationcolony tire newport newsNettetbisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection … colony tire corporate office