Gfp-trap®_a coupled to agarose
http://www.bio-protech.com.tw/upload/20240821120256.pdf WebFeb 3, 2024 · The GFP-trap beads immobilized AHA1-GFP 193 proteins were incubated with TMK1 (TMK1KD, WT) or kinase-dead form (TMK1KD,Km) for 1 194 hr. The amounts of H + -ATPase and phosphorylated...
Gfp-trap®_a coupled to agarose
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WebSep 16, 2024 · In this chapter, we describe a method to co-immunoprecipitate the fluorescently tagged candidate protein with its interacting partners from the crude plant cell extracts using green fluorescent protein (GFP)-trap magnetic beads. Key words GFP-trap Protein-protein interaction Co-immunoprecipitation Magnetic beads Download protocol PDF WebJan 14, 2024 · GFP-Trap ® utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of agarose beads. tested on eGFP, GFP, tagGFP, YFP, CFP, Venus, Citrine, AcGFP Content Bead properties Reagent Code Quantity GFP-Trap ® _A gta-20 20 reactions (0.5 ml slurry) GFP-Trap ® _A gta-100 100 reactions (2.5 ml slurry)
Webbe isolated fast and efficiently by immunoprecipitation using the GFP-Trap®. GFP-Trap® utilizes small recombinant alpaca antibody fragments covalently coupled to the surface … WebGFP-Traps utilize super-high affinity alpaca antibody fragments coupled to agarose beads or magnetic agarose beads. These “Nanobody-Traps” are perfect for immuno …
WebI know that the Chromotek GFP TRAP beads are made of agarose, so will boiling them at 95 degrees cause them to melt? If I place the solution back on the magnet, will the beads separate from... Webanti-DYKDDDDK Fab-fragment coupled to agarose beads. Specificity: 1x and 3x DYKDDDDK sequence (1x and 3xFlag ® -tag) at the N-terminus, C-terminus, or internal site of the fusion protein. Applications: IP/Co-IP, MS, protein purification, on-bead enzyme assays, ChIP/RIP analysis Formats: Agarose Shop DYKDDDDK Fab-Trap Nano …
WebArticle Snippet: After sonication, the cell debris was pelleted (30 min, at 22000 rpm, at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads ( ChromoTek) for 1 hr at 4°C. Figure Lengend Snippet: a) Anti-GFP immunoblot of whole cell extracts from H99, GFP-Bub1 and various GFP-bub1 mutants.
WebIn the past I have used the 9E10 mouse monoclonal antibody cross-linked to Protein G Sepharose beads for IPs and to make an immunoaffinity column to purifiy a myc-tagged protein complex from ... computer learning software for beginnersWebGFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20. Additional Details : Weight : 0.00240kg. View more versions of this product. … computer learning zone reviewWebFisher Scientific - Proteintech The ChromoTek GFP-Trap Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Shop Proteintech ChromoTek GFP … computer learning software for pcWebMouse anti-GFP antibody was used to detect VirE2-Venus protein expressed after induction of individual transgenic lines. The house-keeping protein phosphoenolpyruvate … computer learning center schaumburg ilhttp://www.biotnt.com/news/news_815.html ecmwf hqWebEnter the email address you signed up with and we'll email you a reset link. ecmwf historyWebJul 29, 2024 · For GFP signal, samples were excited at 470/40 nm, and emission range was set at 525/50 nm while for mCherry/RFP signal, samples were excited at 555/30 nm, and emission range was set at 592/25 nm. For simultaneous detection of Nile Red and GFP, imaging was performed using a Leica SP5 confocal microscope and a Leica HCX 63 × … computer learning games for kids free