B-tubulin molecular weight
WebTubulin is a heterodimer, which consists of a-tubulin and b-tubulin; [ 4] both subunits have a molecular weight of 50,000 and share considerable homology. Specificity The antibody has been used to detect acetylated α … WebSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Protocol …
B-tubulin molecular weight
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Webbeta Tubulin Antibody (32-2600) in ICC/IF Immunofluorescent analysis of Beta - Tubulin was done on 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. WebDec 5, 2024 · LWJ-M30 also dramatically decreased the level of polymeric tubulin, while the disruption of microtubules caused the cell cycle to be arrested in the G2/M phase. ... DM1-HKD with correct molecular weight and high purity (>98%) was obtained, which was confirmed by ESI-MS and analytical HPLC (Fig. 1B and C).
WebUse a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa). ... The cells were then incubated with ab16051 at 1μg/ml and ab7291 (staining Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary ... WebApr 9, 2024 · N-terminal myristoylated, but not unmyristoylated, HIV-1 Nef associates with actin in human B and T lymphocytes forming a high-molecular-mass complex of 150 …
WebAlpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa. Tyrosine ligase adds a … WebTubulin, the subunit protein of microtubules, is a dimer that sediments at 6 S and has a molecular weight of 110,000. Using high resolution polyacrylamide gel electrophoresis, …
WebThis antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
WebSpecificity The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. support nba2kWebbeta Tubulin Antibody (PA1-41331) in ICC/IF Immunofluorescence analysis of beta tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. support na kwadratWebOur previous study found that 2-phenyl-4-quinolone (2-PQ) derivatives are antimitotic agents, and we adopted the drug design concept of scaffold hopping to replace the 2-aromatic ring of 2-PQs with a 4-aromatic ring, representing 4-phenyl-2-quinolones (4-PQs). The 4-PQ compounds, whose structural backbones also mimic analogs of … support neeko buildWebIts molecular weight is approximately 42 kDa. References [ edit] ^ a b c GRCh38: Ensembl release 89: ENSG00000075624 - Ensembl, May 2024 ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029580 - Ensembl, May 2024 ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. barberan supportWebSelecting the right loading control antibody is determined by the molecular weight of the other targets being studied, subcellular fraction of the sample, and the cell and tissue types being evaluated. You need to trust that your loading control is … barberan songWebEach has a molecular weight of approximately 50 kDa. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. The β-tubulin … barber anti fatigue mat ukWebAll lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab18251) at 0.5 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate Lane 4 : Caco-2 (Human colonic carcinoma cell … barberan spain